Effective machine-learning assembly for next-generation amplicon sequencing with very low coverage
نویسندگان
چکیده
منابع مشابه
Assembly algorithms for next-generation sequencing data.
The emergence of next-generation sequencing platforms led to resurgence of research in whole-genome shotgun assembly algorithms and software. DNA sequencing data from the Roche 454, Illumina/Solexa, and ABI SOLiD platforms typically present shorter read lengths, higher coverage, and different error profiles compared with Sanger sequencing data. Since 2005, several assembly software packages hav...
متن کاملParsing ecological signal from noise in next generation amplicon sequencing
It is clear that the use of next generation sequencing (NGS) applied to environmental DNA is changing the way researchers conduct experiments and significantly deepening our understanding of microbial communities around the globe (Amend et al., 2010; Caporaso et al., 2011; Bik et al., 2012; Bates et al., 2013). The lower per unit cost and sheer number of sequences relative to traditional method...
متن کاملParsing ecological signal from noise in next generation amplicon sequencing.
It is clear that the use of next generation sequencing (NGS) applied to environmental DNA is changing the way researchers conduct experiments and significantly deepening our understanding of microbial communities around the globe (Amend et al., 2010; Caporaso et al., 2011; Bik et al., 2012; Bates et al., 2013). The lower per unit cost and sheer number of sequences relative to traditional method...
متن کاملStrategies and Clinical Applications of Next Generation Sequencing
Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
متن کاملLOCAS - a low coverage assembler for next generation sequencing and resequencing data
Within the last five years, a new generation of sequencing technologies has dramatically reduced cost and at the same time increased throughput of genome sequencing. For most application fields these technologies have proven to be good alternatives to the traditional Sanger sequencing although they generate shorter read sequences. For the study of sequence variations like SNPs, indels and longe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2019
ISSN: 1471-2105
DOI: 10.1186/s12859-019-3287-2